Journal: Neuropsychiatric Disease and Treatment

Article Title: Investigation on Inhibiting SPHK - S1P Pathway to Improve ADHD Model Rats Behavior via Lipidomics

doi: 10.2147/NDT.S530564

Figure Lengend Snippet: Changes in gene and protein expression following SKI II intervention ( A ) Relative expression levels of Sphk1 mRNA. ( B ) Relative expression levels of Sphk2 mRNA. ( C ) Representative Western blot bands showing SPHK1/GAPDH and SPHK2/GAPDH ratios. ( D ) Relative protein levels of SPHK1. ( E ) Relative protein levels of SPHK2. ( F ) S1P levels in the PFC were measured by ELISA. ( G ) Representative Western blot bands showing DBH/GAPDH, MAOA/GAPDH, TH/GAPDH, and DRD1/GAPDH ratios. ( H ) Relative protein levels of DBH. Each value represents the mean ± standard deviation of at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Primary antibodies were incubated overnight at 4°C in the following concentrations: GAPDH (1:10,000; Proteintech, China), β-Tubulin (1:4000; Proteintech), CERK (1:1000; ABclonal, China), CERS1 (1:1000; Huamei Bio, China), SMPD1 (1:1500; ABclonal), SGMS1 (1:1000; ABclonal), HEXA (1:700; ABclonal), SPHK1 (1:1000; Proteintech), SPHK2 (1:1000; Wanlei Bio), DBH (1:500; ABclonal), DRD1 (1:700; Proteintech), MAOA (1:1000; Proteintech), and TH (1:2000; Proteintech).

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Standard Deviation